December 03, 2020 /
— 4:00 pm
Online keynote lecture with Dr. Philipp Baaske followed by Q&A
In our event series "Life Science Entrepreneurs Talk", start-ups from the life sciences present themselves at regular intervals to talk about their product or process. Interested people and founders get an insight into the development of the start-up and receive helpful tips and tricks in the Life Science Entrepreneurs environment. We are happy to gain insights into the development of NanoTemper on December 3rd and invite all interested participants to register for the event!
The journey for NanoTemper began in the late 2000s, when Philipp and Stefan were working as PhD students at Ludwig-Maximilian University Munich. They were working in the Department of Physics and Center for NanoScience. Stefan originally came from a biochemistry background, while Philipp had been working in the world of physics for some time. Even within their lab, their paths didn’t collide until they worked together on a project that aimed to do super-fast measurements on dsDNA unfolding. This project required a fluorophore on the DNA and a laser that heated their samples up to 110°C very rapidly. (Anyone familiar with NanoTemper technology might see where this is going…)
Philipp and Stefan were successful with their research project, but this success prompted them to think further—how could they apply this cool technology to bench research? They found their technology was good for rapidly analyzing nucleic acid unfolding. However, though their process saved time, researchers working with nucleic acids often had to wait for time-consuming PCR protocols to amplify their product, and shaving a few minutes off the end of their process wasn’t of much practical use. Saving time on the unfolding characterization simply was not all that helpful, even if using a fluorophore to do it was novel and interesting.
Fortunately, Philipp and Stefan applied the principles of heat changes and fluorophores to a new realm: protein biophysics. With Stefan’s biochemical background and Philipp’s physics and engineering understanding, plus a little help from Charles Soret’s 1879 theory of thermodiffusion, they found a way to measure the strength of interactions in solution.
There was an obvious and immediate need for this technology. Researchers in the world of protein biophysics had long been using SPR, ITC, and fluorescence polarization to measure interactions between biological molecules, but there were drawbacks to these methods—sample consumption, immobilization, buffer limitations, etc. Philipp and Stefan’s new technology was not limited in this way, and they had developed a system that was incredibly precise at raising temperatures of small sample volumes only a few degrees.
Anyone who would like to know how the story continues and what tips the founder Phillip Baaske has in store for start-ups is cordially invited to find out in our Leading Life Science Entrepreneurs Talk. Of course, there will be the opportunity to ask individual questions to our speaker.